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ProductsBack/Rat Anti-Mouse Foxp3-AF647
Fixed and permeabilized BALB/c splenocytes were stained with Rat Anti-Mouse Foxp3-AF647 (SB Cat. No. 12400-31), Rat Anti-Mouse CD4-FITC (SB Cat. No. 1540-02), and Rat Anti-Mouse CD25-PE (SB Cat. No. 1595-09). A - CD4 vs Foxp3 gated on small lymphocytes B - CD25 vs Foxp3 gated on CD4+ cells
Fixed and permeabilized BALB/c splenocytes were stained with Rat Anti-Mouse Foxp3-AF647 (SB Cat. No. 12400-31), Rat Anti-Mouse CD4-FITC (SB Cat. No. 1540-02), and Rat Anti-Mouse CD25-PE (SB Cat. No. 1595-09). A - CD4 vs Foxp3 gated on small lymphocytes B - CD25 vs Foxp3 gated on CD4+ cells
Fixed and permeabilized BALB/c splenocytes were stained with Rat Anti-Mouse Foxp3-AF647 (SB Cat. No. 12400-31), Rat Anti-Mouse CD4-FITC (SB Cat. No. 1540-02), and Rat Anti-Mouse CD25-PE (SB Cat. No. 1595-09). A - CD4 vs Foxp3 gated on small lymphocytes B - CD25 vs Foxp3 gated on CD4+ cells

Rat Anti-Mouse Foxp3-AF647

12400-31
SouthernBiotech
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse
TargetFoxp3
0.1 mg
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Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Rat Anti-Mouse Foxp3-AF647
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SB168a
  • Conjugate
    AlexaFluor 647
  • Gene ID20371
  • Target name
    Foxp3
  • Target description
    forkhead box P3
  • Target synonyms
    JM2, scurfin, sf, forkhead box protein P3, scurfy
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDQ99JB6
  • Protein Name
    Forkhead box protein P3
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Foxp3 using anti-Foxp3 antibody (A00011-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Foxp3 antigen affinity purified polyclonal antibody (Catalog # A00011-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Foxp3 at approximately 50 kDa. The expected band size for Foxp3 is at 50 kDa.
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