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RCC1 Antibody (YA2642, Rabbit)

HY-P82897
MedChem Express
TargetRCC1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    RCC1 Antibody (YA2642, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1104
  • Target name
    RCC1
  • Target description
    regulator of chromosome condensation 1
  • Target synonyms
    CHC1, IIAAN, RCC1-I, regulator of chromosome condensation, cell cycle regulatory protein, guanine nucleotide-releasing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    RCC1 Antibody (YA2642) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2642), targeting RCC1, with a predicted molecular weight of 45 kDa (observed band size: 45 kDa). RCC1 Antibody (YA2642) can be used for WB, IHC-P, ICC/IF, IP, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RCC1 using anti-RCC1 antibody (A02719-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RCC1 antigen affinity purified polyclonal antibody (Catalog # A02719-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RCC1 at approximately 45KD. The expected band size for RCC1 is at 45KD.
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