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Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA822281ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA822281ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA822281ESR2HU at dilution of 1:100

RCHY1 Antibody

CSB-PA822281ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRCHY1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RCHY1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25898
  • Target name
    RCHY1
  • Target description
    ring finger and CHY zinc finger domain containing 1
  • Target synonyms
    androgen-receptor N-terminal-interacting protein; ARNIP; CHIMP; CH-rich interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; p53-induced protein with a RING-H2 domain; PIRH2; PRO1996; ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase; RING finger and CHY zinc finger domain-containing protein 1; RING finger protein 199; RING-type E3 ubiquitin transferase RCHY1; RNF199; ZCHY; zinc finger protein 363; zinc finger, CHY-type; ZNF363
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PM5
  • Protein Name
    RING finger and CHY zinc finger domain-containing protein 1
  • Scientific Description
    Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Pirh2/RCHY1 using anti-Pirh2/RCHY1 antibody (A04533-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pirh2/RCHY1 antigen affinity purified polyclonal antibody (Catalog # A04533-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pirh2/RCHY1 at approximately 21 kDa. The expected band size for Pirh2/RCHY1 is at 30 kDa.
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