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Western blot analysis of RET (arrow) using rabbit polyclonal RET Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RET gene (Lane 2).
Western blot analysis of RET (arrow) using rabbit polyclonal RET Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RET gene (Lane 2).
Western blot analysis of RET (arrow) using rabbit polyclonal RET Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RET gene (Lane 2).

RET (C-term) Rabbit Polyclonal Antibody

AP14411PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRET
Price on request
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    RET (C-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5979
  • Target name
    RET
  • Target description
    ret proto-oncogene
  • Target synonyms
    CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET-ELE1, proto-oncogene tyrosine-protein kinase receptor Ret, RET receptor tyrosine kinase, cadherin family member 12, cadherin-related family member 16, proto-oncogene c-Ret, rearranged during transfection, ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
  • Host
    Rabbit
  • Protein IDP07949
  • Protein Name
    Proto-oncogene tyrosine-protein kinase receptor Ret
  • Scientific Description
    RET (C-term) rabbit polyclonal antibody, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RET using anti-RET antibody (A00293-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates Lane 2: human HL-60 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RET antigen affinity purified polyclonal antibody (Catalog # A00293-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RET at approximately 170KD. The expected band size for RET is at 124KD.
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Anti-RET Antibody Picoband(r)A00293-1-CARRIER-FREE
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ReactivityHuman, Mouse, Rat
TargetRET
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Ret antibody70R-RG002
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Biomatik
Ret Recombinant AntibodyCAC12550
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Ret Polyclonal AntibodyCAB0018
ReactivityHuman
TargetRET
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Paraformaldehyde-fixed, paraffin embedded rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RET Polyclonal Antibody, Unconjugated (bs-2793R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
Bioss
RET Polyclonal AntibodyBS-2793R
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ReactivityCanine, Human, Mouse, Rabbit, Rat
TargetRET
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Western Blot analysis of various cells using Ret Polyclonal Antibody
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ReactivityHuman, Mouse, Rat
TargetRET
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Product group Antibodies
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RET AntibodyLS-C761146
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Anti-RET Antibody
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Immunohistochemical staining of human adrenal gland shows strong membranous positivity in glandular cells.
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