Immunoprecipitation of RFC1 protein from HeLa whole cell extracts using 5 μg of RFC1 antibody (GTX129292). Western blot analysis was performed using RFC1 antibody (GTX129292) diluted at 1:1500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of RFC1 protein from HeLa whole cell extracts using 5 μg of RFC1 antibody (GTX129292). Western blot analysis was performed using RFC1 antibody (GTX129292) diluted at 1:1500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
RFC1 antibody
GTX129292
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetRFC1
Overview
- SupplierGeneTex
- Product NameRFC1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5981
- Target nameRFC1
- Target descriptionreplication factor C subunit 1
- Target synonymsA1, CANVAS, MHCBFB, PO-GA, RECC1, RFC, RFC140, replication factor C subunit 1, A1 140 kDa subunit, DNA-binding protein PO-GA, MHC binding factor, beta, RF-C 140 kDa subunit, activator 1 140 kDa subunit, activator 1 large subunit, activator 1 subunit 1, replication factor C (activator 1) 1, 145kDa, replication factor C 140 kDa subunit, replication factor C large subunit, replication factor C1
- HostRabbit
- IsotypeIgG
- Protein IDP35251
- Protein NameReplication factor C subunit 1
- Scientific DescriptionThe protein encoded by this gene is the large subunit of replication factor C, which is a five subunit DNA polymerase accessory protein. Replication factor C is a DNA-dependent ATPase that is required for eukaryotic DNA replication and repair. The protein acts as an activator of DNA polymerases, binds to the 3 end of primers, and promotes coordinated synthesis of both strands. It also may have a role in telomere stability. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RFC1 antibody [HL3242] (GTX640885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640885/GTX640885_T-45516_20240830_WB_24090423_123.webp)
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