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ProductsBack/RIP (phospho Tyr384) antibody
ELISA analysis of phospho- and non phospho- immunogen peptide using GTX03682 RIP (phospho Tyr384) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml
ELISA analysis of phospho- and non phospho- immunogen peptide using GTX03682 RIP (phospho Tyr384) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml
ELISA analysis of phospho- and non phospho- immunogen peptide using GTX03682 RIP (phospho Tyr384) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1microg/ml

RIP (phospho Tyr384) antibody

GTX03682
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRIPK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RIP (phospho Tyr384) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8737
  • Target name
    RIPK1
  • Target description
    receptor interacting serine/threonine kinase 1
  • Target synonyms
    AIEFL, IMD57, RIP, RIP-1, RIP1, receptor-interacting serine/threonine-protein kinase 1, cell death protein RIP, receptor (TNFRSF)-interacting serine-threonine kinase 1, receptor-interacting protein 1, receptor-interacting protein kinase 1, serine/threonine-protein kinase RIP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13546
  • Protein Name
    Receptor-interacting serine/threonine-protein kinase 1
  • Scientific Description
    This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RIP using anti-RIP antibody (PB9116). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human RIP protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 38 kDa. The expected band size for RIP is at 38 kDa.
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