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Ripk1 Polyclonal Antibody

CAC09275
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetRIPK1
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Overview

  • Supplier
    Biomatik
  • Product Name
    RIPK1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:200-1:500
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8737
  • Target name
    RIPK1
  • Target description
    receptor interacting serine/threonine kinase 1
  • Target synonyms
    AIEFL; cell death protein RIP; IMD57; receptor (TNFRSF)-interacting serine-threonine kinase 1; receptor-interacting protein 1; receptor-interacting protein kinase 1; receptor-interacting serine/threonine-protein kinase 1; RIP; RIP1; RIP-1; serine/threonine-protein kinase RIP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13546
  • Protein Name
    Receptor-interacting serine/threonine-protein kinase 1
  • Scientific Description
    The Ripk1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IF.
  • Reactivity Supplier
    Human, Rat, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Raji cell lysates probed with RIPK-1 Polyclonal Antibody, Unconjugated (bs-5805R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of RIP using anti-RIP antibody (PB9116). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human RIP protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 38 kDa. The expected band size for RIP is at 38 kDa.
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