More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/RNF8 antibody, N-term
GTX15850 staining (0.1microg/ml) of Human Lung lysate (RIPA buffer, 35microg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
GTX15850 staining (0.1microg/ml) of Human Lung lysate (RIPA buffer, 35microg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
GTX15850 staining (0.1microg/ml) of Human Lung lysate (RIPA buffer, 35microg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

RNF8 antibody, N-term

GTX15850
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetRNF8
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    RNF8 antibody, N-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.1-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9025
  • Target name
    RNF8
  • Target description
    ring finger protein 8
  • Target synonyms
    C3HC4-type zinc finger protein; E3 ubiquitin-protein ligase RNF8; hRNF8; ring finger protein (C3HC4 type) 8; ring finger protein 8, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF8; UBC13/UEV-interacting ring finger protein
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDO76064
  • Protein Name
    E3 ubiquitin-protein ligase RNF8
  • Scientific Description
    The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
Goat anti-RNF8EB05843
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetRNF8
  • SizePrice
Western blot All lanes: RNF8 antibody at 11microg/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 56, 11, 51 kDa Observed band size: 56 kDa
Product group Antibodies
Cusabio
RNF8 AntibodyCSB-PA019898ESR2HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetRNF8
  • SizePrice
Product group Antibodies
Bioss Antibodies
RNF8 Recombinant Antibody, AbBy Fluor-488 ConjugatedBSM-61658R-BF488
ApplicationsWestern Blot
TargetRNF8
  • SizePrice
Anti-RNF8 Antibody
Product group Antibodies
Antibodies.com
Anti-RNF8 AntibodyA10107
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
References
GeneTex
RNF8 antibody [N3C3]GTX115176
ApplicationsWestern Blot
TargetRNF8
  • SizePrice
Product group Antibodies
MedChem Express
RNF8 Antibody (YA1344)HY-P81599
TargetRNF8
  • SizePrice
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.
Product group Antibodies
Atlas Antibodies
Anti-RNF8 AntibodyHPA064925
ApplicationsImmunoCytoChemistry
TargetRNF8
  • SizePrice
WB analysis of HepG2 cell lysate using GTX55783 RNF8 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
RNF8 antibodyGTX55783
ApplicationsWestern Blot
TargetRNF8
  • SizePrice
Figure 1. Western blot analysis of RNF8 using anti-RNF8 antibody (A00707-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF8 antigen affinity purified polyclonal antibody (Catalog # A00707-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF8 at approximately 56 kDa. The expected band size for RNF8 is at 56 kDa.
Product group Antibodies
Boster Bio
Anti-RNF8 Antibody Picoband(r)A00707-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetRNF8
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RNF8 Antibody144-63065
ApplicationsImmunoFluorescence, Western Blot
TargetRNF8
  • SizePrice