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Immunofluorescence analysis of U-2 OS cells using ROBO1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ROBO1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ROBO1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ROBO1 Polyclonal Antibody

E-AB-65262
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ROBO1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ROBO1 using anti-ROBO1 antibody (A01530-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO1 antigen affinity purified polyclonal antibody (Catalog # A01530-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO1 at approximately 250 kDa. The expected band size for ROBO1 is at 181 kDa.
Product group Antibodies
Boster Bio
Anti-ROBO1 Antibody Picoband(r)A01530-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetROBO1
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Product group Antibodies
Absolute Antibody
Anti-ROBO-1 [B2212A]Ab00398-1.1
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ReactivityHuman
TargetROBO1
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Product group Antibodies
RayBiotech
Anti-ROBO1 Antibody144-60992
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ReactivityHuman, Mouse, Rat
TargetROBO1
  • SizePrice
Product group Antibodies
Biomatik
Robo1 Polyclonal AntibodyCAC11061
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetROBO1
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Tissue: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; \nAntigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Blocking buffer (normal goat serum 5%) at 37℃ for 20 min; \nIncubation: Anti-Robo Polyclonal Antibody, Unconjugated (bs-5794R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.
Product group Antibodies
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Bioss
Robo Polyclonal AntibodyBS-5794R
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ReactivityHuman, Mouse, Rat
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  • SizePrice
Product group Antibodies
MedChem Express
Anti-ROBO1 AntibodyHY-P990500
  • SizePrice
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
Product group Antibodies
Atlas Antibodies
Anti-ROBO1 AntibodyHPA052968
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Everest Biotech
Goat anti-ROBO1 / DUTT1 (Internal) AntibodyEB07058
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TargetROBO1
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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: ROBO1 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 181, 182, 177, 176, 170 kDa Observed band size: 181 kDa
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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO1 antibody [C2C3], C-term (GTX113826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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ROBO1 antibody [C2C3], C-termGTX113826
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