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ProductsBack/SATB1 Mouse Monoclonal Antibody [Clone ID: OTI6C6]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1. Positive lysates LY418976 (100ug) and LC418976 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1. Positive lysates LY418976 (100ug) and LC418976 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (RC200421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1. Positive lysates LY418976 (100ug) and LC418976 (20ug) can be purchased separately from OriGene.

SATB1 Mouse Monoclonal Antibody [Clone ID: OTI6C6]

CF500547
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSATB1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SATB1 Mouse Monoclonal Antibody [Clone ID: OTI6C6]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI6C6
  • Gene ID6304
  • Target name
    SATB1
  • Target description
    SATB homeobox 1
  • Target synonyms
    DEFDA, DHDBV, KTZSL, DNA-binding protein SATB1, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ01826
  • Protein Name
    DNA-binding protein SATB1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SATB1 mouse monoclonal antibody, clone OTI6C6 (formerly 6C6)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SATB1 using anti-SATB1 antibody (A01312-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SATB1 antigen affinity purified polyclonal antibody (Catalog # A01312-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SATB1 at approximately 100KD. The expected band size for SATB1 is at 86KD.
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Immunohistochemical staining of human thymus shows strong nuclear positivity in cortical cells.
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Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SATB1 (Ser47) Polyclonal Antibody(bs-3390R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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