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Search results: Histone H3/currency/EUR

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ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 microl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and ALDOA and for the coding region of the myogenic differentiation gene (MYOD). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP gradeGTX60372
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Yeast
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60817 Histone H3K36me3 (Tri-methy Lys36) antibody - ChIP grade. A titration consisting of 0.2, 0.5, 1 and 2 microg of antibody per ChIP experiment was analyzed. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the coding region of the inactive MB gene and the Sat satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3K36me3 (Tri-methy Lys36) antibody - ChIP gradeGTX60817
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, Other Application
ReactivityHuman, Mouse, Plant
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 10? K562 cells using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 microg of antibody per ChIP experiment was analyzed. IgG (1 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 gene, respectively, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
References
GeneTex
Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP gradeGTX60818
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, Other Application
ReactivityHuman, Mouse
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 5x10? HeLaS3 cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 microg per ChIP experiment was analysed. IgG (2 microg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for a region upstream of the ACTB and GAS2L1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, H3K36me2, H4K20me2 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me2 modification.
Product group Antibodies
GeneTex
Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP gradeGTX60819
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
ReactivityHuman, Plant
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 5x10?HeLa cells using GTX60821 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. IgG (5 microg/IP) was used as a negative IP control. The IPd DNA was analysed by qPCR using primers for different positive and negative loci. The results are expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1A: recovery of the GAPDH promoter and myoglobin exon 2 with a titration of the H3K79me1 antibody consisting of 5, 10 and 15 microl per ChIP experiment. Figure 1B: recovery of RPL30, ALDOA, SERPINA1 and SAA4 using 10 microl of antibody per ChIP experiment.
Product group Antibodies
GeneTex
Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP gradeGTX60821
ApplicationsDot Blot, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
ReactivityHuman
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 microg of antibody per ChIP experiment was analyzed. IgG (2 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP gradeGTX60822
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, Other Application
ReactivityFungus, Human, Mouse, Plant, Zebra Fish, Other Species
TargetH3C1
  • SizePrice
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60824 Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 microg of antibody per ChIP experiment was analyzed. IgG (2 microg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as negative controls, and for ZNF510 and the Sat2 satellite repeat, used as positive controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Product group Antibodies
GeneTex
Histone H3K9me3 (Tri-methyl Lys9) antibody - ChIP gradeGTX60824
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, Other Application
ReactivityHuman, Mouse, Yeast
TargetH3C1
  • SizePrice
Product group Antibodies
Biorbyt
Histone H2B(Di-Methyl-Lys5) Rabbit Polyclonal AntibodyORB251806
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH2BC3
  • SizePrice
Product group Antibodies
Biorbyt
Histone H2B(Tri-Methyl-Lys5) Rabbit Polyclonal AntibodyORB251811
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH2BC3
  • SizePrice
Product group Antibodies
Biorbyt
Histone H2B(mono-Methyl-Lys5) Rabbit Polyclonal AntibodyORB251805
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH2BC3
  • SizePrice
Product group Antibodies
Biorbyt
Histone H2B(Di-Methyl-Lys43) Rabbit Polyclonal AntibodyORB251807
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH2BC3
  • SizePrice
Product group Antibodies
Biorbyt
Histone H2B(Tri-Methyl-Lys43) Rabbit Polyclonal AntibodyORB251808
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH2BC3
  • SizePrice
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