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Search results: IL17B

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Immunohistochemical analysis of paraffin-embedded human-small-intestine, antibody was diluted at 1:200
Product group Antibodies
Cusabio
IL17B AntibodyCSB-PA648539
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetIL17B
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Product group Molecular Biology
Sino Biological
Mouse, IL17B cDNA ORF Clone, untaggedMG51304-UT
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Product group Proteins / Signaling Molecules
Sino Biological
Recombinant Mouse IL17B Protein (hFc Tag)51304-M02B
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Product group Proteins / Signaling Molecules
Sino Biological
Recombinant Human IL17B Protein (hFc Tag)11939-H02B
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Product group Molecular Biology
Sino Biological
Human, IL17B cDNA ORF Clone, untaggedHG11939-UT
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Product group Molecular Biology
Sino Biological
Rat, IL17B cDNA ORF Clone, untaggedRG80170-UT
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Product group Molecular Biology
Sino Biological
Mouse IL17B Baculovirus-Insect cells Overexpression Lysate51304-M02BL
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Immunohistochemical staining of human spleen shows moderate nuclear positivity in cells in red pulp.
Product group Antibodies
Atlas Antibodies
Anti-IL17B AntibodyHPA042441
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetIL17B
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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat gaster tissue lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 17KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B AntibodyA10846-2
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL17B
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Figure 1. Western blot analysis of IL17B using anti-IL17B antibody (A10846-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17B antigen affinity purified polyclonal antibody (Catalog # A10846-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17B at approximately 20KD. The expected band size for IL17B is at 20KD.
Product group Antibodies
Boster Bio
Anti-IL17B AntibodyA10846-3
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRNU4-7P
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Product group Proteins / Signaling Molecules
Atlas Antibodies
PrEST Antigen IL17BAPREST74079
Protein IDQ9UHF5
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Product group Antibodies
Abbexa
Interleukin 17B (IL17B) AntibodyABX038115
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
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