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Search results: RAF1

421 to 432 of 2.195 products
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Product group Antibodies
Biorbyt
Raf1 antibodyORB10460
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat
TargetRAF1
  • SizePrice
Western blot analysis of Phospho-Raf1 (S43) expression in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate (P00446-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody (Catalog # P00446-3) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAF1
Product group Antibodies
Boster Bio
Anti-Phospho-Raf1 (S43) Rabbit Monoclonal AntibodyP00446-3
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
Western blot analysis of Phospho-Raf1 (S621) expression in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate (P00446-4).
Product group Antibodies
Boster Bio
Anti-Phospho-Raf1 (S621) Rabbit Monoclonal AntibodyP00446-4
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.
Product group Antibodies
Boster Bio
Anti-Phospho-Raf1 (S259) Rabbit Monoclonal AntibodyP00446-5
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAF1
WB analysis of various samples using GTX01196 Raf1 antibody [GT1215]. Dilution : 1:1000 Loading : 25microg
Product group Antibodies
GeneTex
Raf1 antibody [GT1215] - Recombinant antibody, Orthogonal ValidatedGTX01196
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
  • SizePrice
Untreated (-) and treated (+) 293T whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Raf1 (phospho Ser259) antibody [GT1309] (GTX03221) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
Raf1 (phospho Ser259) antibody [GT1309] - Orthogonal ValidatedGTX03221
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetRAF1
  • SizePrice
WB analysis of immunoprecipitates prepared HEK293 cells overexpressing wild-type c-Raf and stimulated with EGF (Lane 1-4) or c-Raf mutant S621A (Lane 5) using GTX24767 Raf1 (phospho Ser621) antibody.
Product group Antibodies
References
GeneTex
Raf1 (phospho Ser621) antibody - Orthogonal, Overexpression ValidatedGTX24767
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Rat
TargetRAF1
  • SizePrice
WB (peptide competition) analysis of HEK293 cells overexpressing c-Raf unstimulated (Lane 1) or stimulated with active Src (Lane 2-5) using GTX24769 Raf1 (phospho Tyr340/Tyr341) antibody prior incubated with non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
Product group Antibodies
GeneTex
Raf1 (phospho Tyr340/Tyr341) antibody - Orthogonal ValidatedGTX24769
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAF1
  • SizePrice
Product group Proteins / Signaling Molecules
Sino Biological
RAF1 Protein, Human, Recombinant (His & GST Tag)10657-H20B
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
RAF1 cDNA ORF Clone, Human, untaggedHG10657-UT
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
RAF1 cDNA ORF Clone, Mouse, untaggedMG50287-UT
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RAF1 / RAF Antibody (aa342-619, APC, Cy7)LS-C734252
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice
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