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Search results: Siglec-3

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Western blot analysis of Aromatase expression in rat brain lysate.
Product group Antibodies
Boster Bio
Anti-Aromatase CYP19A1 Rabbit Monoclonal AntibodyM00071
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Rat
TargetCYP19A1
  • SizePrice
Western blot analysis of pro Caspase 9 expression in Jurkat cell lysate (M00080-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP9 monoclonal antibody (Catalog # M00080-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP9
Product group Antibodies
Boster Bio
Anti-pro Caspase 9 CASP9 Rabbit Monoclonal AntibodyM00080-1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCASP9
  • SizePrice
Western blot analysis of cleaved Caspase-9 Antibody expression in HeLa cell lysate treated with staurosporine (M00080-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP9 monoclonal antibody (Catalog # M00080-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP9
Product group Antibodies
References
Boster Bio
Anti-cleaved Caspase-9 CASP9 Rabbit Monoclonal AntibodyM00080-2
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse
TargetCASP9
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human liver cancer, using Caspase-9 Antibody(M00080-3) CASP9 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP9 Antibody (M00080-3)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-Caspase-9 CASP9 Rabbit Monoclonal AntibodyM00080-3
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCASP9
  • SizePrice
Western blot analysis of Caspase-9 expression in HeLa cell lysate.
Product group Antibodies
References
Boster Bio
Anti-Caspase-9 CASP9 Rabbit Monoclonal AntibodyM00080
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCASP9
  • SizePrice
Western blot analysis of CETP expression in human plasma lysate.
Product group Antibodies
Boster Bio
Anti-CETP Rabbit Monoclonal AntibodyM00309
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetCETP
  • SizePrice
Western blot analysis of CYBB expression in MCF-7 cell lysate (M00328). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYBB monoclonal antibody (Catalog # M00328) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYBB
Product group Antibodies
Boster Bio
Anti-CYBB/Nox2 Rabbit Monoclonal AntibodyM00328
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCYBB
  • SizePrice
Western blot analysis of pro Caspase 3 expression in Jurkat cell lysate (M00334-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3
Product group Antibodies
Boster Bio
Anti-pro Caspase 3 CASP3 Rabbit Monoclonal AntibodyM00334-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCASP3
  • SizePrice
Western blot analysis of Caspase-3 p12 expression in HeLa cell treated with staurosporine lysate (M00334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3
Product group Antibodies
References
Boster Bio
Anti-Caspase-3 p12 CASP3 Rabbit Monoclonal AntibodyM00334-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCASP3
  • SizePrice
Western blot analysis of active Caspase-3 expression in Jurkat cell lysate treated with Camptothecin.
Product group Antibodies
Boster Bio
Anti-active Caspase-3 CASP3 Rabbit Monoclonal AntibodyM00334-3
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCASP3
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human stomach, using active + pro Caspase 3 Antibody (M00334-4) CASP3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP3 Antibody (M00334-4)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-active + pro Caspase 3 CASP3 Rabbit Monoclonal AntibodyM00334-4
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCASP3
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human tonsil, using Caspase-3 Antibody(M00334-5) CASP3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CASP3 Antibody (M00334-5)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-Caspase-3 CASP3 Rabbit Monoclonal AntibodyM00334-5
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCASP3
  • SizePrice
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