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Search results: Siglec-3

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Western blot analysis of pro Caspase 3 in HeLa cell lysate (M00334). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody (Catalog # M00334) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP3
Product group Antibodies
References
Boster Bio
Anti-pro Caspase 3 CASP3 Rabbit Monoclonal AntibodyM00334
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCASP3
  • SizePrice
Western blot analysis of CYP3A4 using anti-CYP3A4 antibody (M00339) in Human fetal liver lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP3A4 antigen affinity purified polyclonal antibody (Catalog # M00339) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal AntibodyM00339
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCYP3A4
  • SizePrice
Western blot analysis of CD4 expression in THP-1 cell lysate (M00344). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 monoclonal antibody (Catalog # M00344) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4
Product group Antibodies
Boster Bio
Anti-CD4 Rabbit Monoclonal AntibodyM00344
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD4
  • SizePrice
Western blot analysis of CEA(CD66e) expression in Human colon cancer lysate (M00356-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 monoclonal antibody (Catalog # M00356-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal AntibodyM00356-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCEACAM5
  • SizePrice
Western blot analysis of CDK6 expression in HeLa cell lysate (M00358). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK6 monoclonal antibody (Catalog # M00358) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK6
Product group Antibodies
Boster Bio
Anti-CDK6 Rabbit Monoclonal AntibodyM00358
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCDK6
  • SizePrice
Western blot analysis of extracts of NIH/3T3 cell lysate, using CD47 antibody.
Product group Antibodies
References
Boster Bio
Anti-CD47 Rabbit Monoclonal AntibodyM00360
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCD47
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human colon, using CD46 Antibody.
Product group Antibodies
Boster Bio
Anti-CD46 Rabbit Monoclonal AntibodyM00377-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD46
  • SizePrice
Western blot analysis of CEBP alpha expression in U937 cell lysate (M00386). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPA monoclonal antibody (Catalog # M00386) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBPA
Product group Antibodies
Boster Bio
Anti-CEBP alpha Rabbit Monoclonal AntibodyM00386
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetCEBPA
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human spleen, using CCR7 Antibody.
Product group Antibodies
Boster Bio
Anti-CCR7 Rabbit Monoclonal AntibodyM00390
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR7
  • SizePrice
Western blot analysis of Nav1.2 on rat brain membrane tissues using a 1:1000 dilution of the antibody
Product group Antibodies
OriGene
Scn2a Mouse Monoclonal Antibody [Clone ID: S69-3]TA326577
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetScn2a
  • SizePrice
Product group Antibodies
Biorbyt
CD3 CD3E Antibody (Monoclonal, CA-3)ORB18193
ReactivityHuman
TargetCd3e
  • SizePrice
Product group Antibodies
Biorbyt
Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3)ORB18212
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetCcnd2
  • SizePrice
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