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Mouse NLRP1b (mouse) (full-length and UFA-CARD fragment) is detected in Raw264.7 cells using NLRP1b, mAb (2A12) (Prod. No. AG-20B-0084). Method: Cell extracts (about 30microg) from the mouse cells Raw264.7 (lane 1) or Raw264.7 NLRP1b KO (lane 2) are separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the NLRP1b, mAb (2A12) (1microg/ml). After incubation with anti-mouse Alexa 633 conjugated antibody, proteins are visualized by a fluorescence detection system at 700nm. Picture Courtesy of Dr Andrew Sandstrom, UT Southwestern Medical Center.
Product group Antibodies
AdipoGen Life Sciences
anti-NLRP1b (mouse), mAb (2A12)AG-20B-0084
ApplicationsWestern Blot
ReactivityMouse, Rat
Figure 1: Immunoblot analysis of beta-tubulin protein glutamylation using anti-beta-Tubulin, mAb (AXO45) (Prod. No. AG-20B-0085). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the different glutamylases, TTLL4 and TTLL6 and are run on a 10% SDS-PAGE (specific protocol to separate alpha- and beta-tubulin (Magiera & Janke, 2013)). The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the AXO45 (1:1000) in PBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In all the cell extracts, AXO45 specifically detects beta-tubulin, with no cross-reactivity to alpha-tubulin. Glutamylation added by either TTLL4 or TTLL6 appears to not affect this detection. Picture courtesy of Sudarshan Gadadhar, Shreyangi Chakraborty, Mariya Genova and Carsten Janke, Institut Curie, Orsay.
Product group Antibodies
AdipoGen Life Sciences
anti-beta-Tubulin, mAb (AXO45)AG-20B-0085
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Other Species
Product group Antibodies
AdipoGen Life Sciences
anti-Periostin, mAb (blocking) (OC-20) (preservative free)AG-20B-6000YPF
ApplicationsNeutralisation/Blocking
Immunohistochemical analysis on paraffin-embedded sections of human melanoma SK-MEL-28 using anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free) (AG-20B-6001PF).
Product group Antibodies
AdipoGen Life Sciences
anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free)AG-20B-6001PF
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
ReactivityBovine, Canine, Chicken, Human, Monkey, Mouse, Porcine, Rat
  • SizePrice
Product group Antibodies
AdipoGen Life Sciences
anti-Fibronectin (EDA), mAb (blocking) (IST-9) (preservative free)AG-20B-6001YPF
ApplicationsNeutralisation/Blocking
Product group Antibodies
AdipoGen Life Sciences
anti-Lewis y/b hapten (human), mAb (SC104) (preservative free)AG-20B-6002PF
ApplicationsFlow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
ReactivityHuman
  • SizePrice
PD-1 receptor-induced CD4 T cell activation and proliferation by PD-1 (mouse), mAb (blocking) (1H10) (AG-20B-0075).Method: Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (b
Product group Antibodies
AdipoGen Life Sciences
anti-PD-1 (human), mAb (AG-IHC001)AG-20B-6020
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPDCD1
  • SizePrice
PD-1 receptor-induced CD4 T cell activation and proliferation by PD-1 (mouse), mAb (blocking) (1H10) (AG-20B-0075).Method: Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (b
Product group Antibodies
AdipoGen Life Sciences
anti-PD-L1 (human), mAb (AG-IHC411)AG-20B-6022
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCD274
  • SizePrice
PD-1 receptor-induced CD4 T cell activation and proliferation by PD-1 (mouse), mAb (blocking) (1H10) (AG-20B-0075).Method: Magnetic bead affinity purified CD4+ T cells from C57BL/6 mice are stimulated in vitro with PD-1 (mouse), mAb (b
Product group Antibodies
AdipoGen Life Sciences
anti-Isocitrate Dehydrogenase 1 [IDH1] (R132H Mutant) (human), mAb (AG-IHC132)AG-20B-6024
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetIDH1
  • SizePrice
anti-Poly(ADP-ribose) [PAR], mAb (10H)
Product group Antibodies
AdipoGen Life Sciences
anti-Poly(ADP-ribose) [PAR], mAb (10H)AG-20T-0001
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityDrosophila, Human, Mouse, Rat
  • SizePrice
Product group Antibodies
AdipoGen Life Sciences
anti-VASP, mAb (IE273)AG-20T-0002
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Porcine, Rabbit, Sheep
TargetVASP
  • SizePrice
Western Blot using anti-Survival Motor Neuron Protein (human), mAb (7B10) (Prod. No. AG-20T-0003) on 15microg of human platelet lysate as a positive control (15microg). ECL detection.
Product group Antibodies
AdipoGen Life Sciences
anti-Survival Motor Neuron Protein, mAb (7B10)AG-20T-0003
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetSMN1
  • SizePrice
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