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ProductsBack/SEMA7A / Semaphorin 7A Antibody

SEMA7A / Semaphorin 7A Antibody

LS-C747248
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSEMA7A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEMA7A / Semaphorin 7A Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 73kDa/74kDa, while the observed MW by Western blot was 80kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 73kDa/74kDa, while the observed MW by Western blot was 80kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8482
  • Target name
    SEMA7A
  • Target description
    semaphorin 7A (JohnMiltonHagen blood group)
  • Target synonyms
    CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, PFIC11, SEMAK1, SEMAL, semaphorin-7A, JMH blood group antigen, John Milton Hagen blood group H-Sema K1, john-Milton-Hargen human blood group Ag, sema K1, sema L, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group), sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A, semaphorin 7A (John Milton Hagen blood group), semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group), semaphorin-K1, semaphorin-L
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Semaphorin 7a/SEMA7A using anti-Semaphorin 7a/SEMA7A antibody (A03832-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Semaphorin 7a/SEMA7A antigen affinity purified polyclonal antibody (Catalog # A03832-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Semaphorin 7a/SEMA7A at approximately 75 kDa. The expected band size for Semaphorin 7a/SEMA7A is at 75 kDa.
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