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ProductsBack/Semaphorin 7A (SEMA7A) Polyclonal Antibody

Semaphorin 7A (SEMA7A) Polyclonal Antibody

CAU25920
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSEMA7A
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Overview

  • Supplier
    Biomatik
  • Product Name
    Semaphorin 7A (SEMA7A) Polyclonal A
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8482
  • Target name
    SEMA7A
  • Target description
    semaphorin 7A (JohnMiltonHagen blood group)
  • Target synonyms
    CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, PFIC11, SEMAK1, SEMAL, semaphorin-7A, JMH blood group antigen, John Milton Hagen blood group H-Sema K1, john-Milton-Hargen human blood group Ag, sema K1, sema L, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group), sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A, semaphorin 7A (John Milton Hagen blood group), semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group), semaphorin-K1, semaphorin-L
  • Host
    Rabbit
  • Protein IDO75326
  • Protein Name
    Semaphorin-7A
  • Scientific Description
    The Semaphorin 7A (SEMA7A) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Semaphorin 7a/SEMA7A using anti-Semaphorin 7a/SEMA7A antibody (A03832-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Semaphorin 7a/SEMA7A antigen affinity purified polyclonal antibody (Catalog # A03832-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Semaphorin 7a/SEMA7A at approximately 75 kDa. The expected band size for Semaphorin 7a/SEMA7A is at 75 kDa.
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