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Formalin-fixed and paraffin embedded rat brain labeled with Anti-SEMA7A/CD108 Polyclonal Antibody, Unconjugated(bs-2702R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-SEMA7A/CD108 Polyclonal Antibody, Unconjugated(bs-2702R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-SEMA7A/CD108 Polyclonal Antibody, Unconjugated(bs-2702R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

CD108 Polyclonal Antibody

BS-2702R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSEMA7A
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Semaphorin 7A Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID8482
  • Target name
    SEMA7A
  • Target description
    semaphorin 7A (John Milton Hagen blood group)
  • Target synonyms
    CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; JMH blood group antigen; John Milton Hagen blood group H-Sema K1; john-Milton-Hargen human blood group Ag; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; sema K1; sema L; SEMAK1; SEMAL; semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group); semaphorin-7A; semaphorin-K1; semaphorin-L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75326
  • Protein Name
    Semaphorin-7A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Semaphorin 7a/SEMA7A using anti-Semaphorin 7a/SEMA7A antibody (A03832-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Semaphorin 7a/SEMA7A antigen affinity purified polyclonal antibody (Catalog # A03832-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Semaphorin 7a/SEMA7A at approximately 75 kDa. The expected band size for Semaphorin 7a/SEMA7A is at 75 kDa.
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