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Western Blot Positive WB detected in: Mouse brain tissue All lanes: SFRP1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: SFRP1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western Blot Positive WB detected in: Mouse brain tissue All lanes: SFRP1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa

SFRP1 Antibody

CSB-PA021138LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSFRP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SFRP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6422
  • Target name
    SFRP1
  • Target description
    secreted frizzled related protein 1
  • Target synonyms
    FRP, FRP-1, FRP1, FrzA, SARP2, secreted frizzled-related protein 1, SARP-2, frizzled-related protein, sFRP-1, secreted apoptosis-related protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N474
  • Protein Name
    Secreted frizzled-related protein 1
  • Scientific Description
    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SFRP1 using anti-SFRP1 antibody (A01968-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP1 antigen affinity purified polyclonal antibody (Catalog # A01968-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP1 at approximately 35KD. The expected band size for SFRP1 is at 35KD.
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FACS analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) using GTX03397 SFRP1 antibody.
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