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SFRP1 Antibody (YA3311, Rabbit)

HY-P83566
MedChem Express
TargetSFRP1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SFRP1 Antibody (YA3311, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6422
  • Target name
    SFRP1
  • Target description
    secreted frizzled related protein 1
  • Target synonyms
    frizzled-related protein; FRP; FRP1; FRP-1; FrzA; SARP2; SARP-2; secreted apoptosis-related protein 2; secreted frizzled-related protein 1; sFRP-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SFRP1 Antibody (YA3311) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3311), targeting SFRP1, with a predicted molecular weight of 35 kDa (observed band size: 35 kDa). SFRP1 Antibody (YA3311) can be used for WB, IHC-P, ICC/IF experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SFRP1 using anti-SFRP1 antibody (A01968-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP1 antigen affinity purified polyclonal antibody (Catalog # A01968-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP1 at approximately 35KD. The expected band size for SFRP1 is at 35KD.
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