More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SFRP1 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SFRP1 Polyclonal Antibody, Unconjugated (bs-1303R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SFRP1 Polyclonal Antibody, Unconjugated (bs-1303R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SFRP1 Polyclonal Antibody, Unconjugated (bs-1303R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

SFRP1 Polyclonal Antibody

BS-1303R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSFRP1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    SFRP1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6422
  • Target name
    SFRP1
  • Target description
    secreted frizzled related protein 1
  • Target synonyms
    FRP, FRP-1, FRP1, FrzA, SARP2, secreted frizzled-related protein 1, SARP-2, frizzled-related protein, sFRP-1, secreted apoptosis-related protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N474
  • Protein Name
    Secreted frizzled-related protein 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot Positive WB detected in: Mouse brain tissue All lanes: SFRP1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Product group Antibodies
Cusabio
SFRP1 AntibodyCSB-PA021138LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSFRP1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-SFRP1EB08864
ApplicationsELISA, ImmunoHistoChemistry
TargetSFRP1
  • SizePrice
Product group Antibodies
MedChem Express
SFRP1 Antibody (YA3311, Rabbit)HY-P83566
TargetSFRP1
  • SizePrice
Product group Antibodies
Biosynth
SFRP1 antibody20R-1319
  • SizePrice
Figure 1. Western blot analysis of SFRP1 using anti-SFRP1 antibody (A01968-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP1 antigen affinity purified polyclonal antibody (Catalog # A01968-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP1 at approximately 35KD. The expected band size for SFRP1 is at 35KD.
Product group Antibodies
Boster Bio
Anti-SFRP1 Antibody Picoband(r)A01968-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetSFRP1
  • SizePrice
Product group Antibodies
Biomatik
SFRP1 Polyclonal AntibodyCAC14649
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetSFRP1
  • SizePrice
Product group Antibodies
Assay Genie
SFRP1 AntibodyCAB2911
TargetSFRP1
  • SizePrice
FACS analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram) using GTX03397 SFRP1 antibody.
Product group Antibodies
GeneTex
SFRP1 antibodyGTX03397
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSFRP1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SFRP1 AntibodyLS-C406022
ApplicationsELISA, ImmunoHistoChemistry
TargetSFRP1
  • SizePrice