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ProductsBack/Sheep anti Human Calreticulin

Sheep anti Human Calreticulin

Z100P
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCALR
250 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Sheep anti Human Calreticulin
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (1:1000-1:5000 dilution) and immunofluorescence (1:50 dilution). Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    Western Blotting;Immunoprecipitation;Immunohistochemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID811
  • Target name
    CALR
  • Target description
    calreticulin
  • Target synonyms
    CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR, calreticulin, CRP55, ERp60, HACBP, Sicca syndrome antigen A (autoantigen Ro; calreticulin), calregulin, endoplasmic reticulum resident protein 60, epididymis secretory sperm binding protein Li 99n, grp60
  • Host
    Sheep
  • Isotype
    IgG
  • Protein IDQ6IAT4
  • Protein Name
    Calreticulin
  • Shelf life instruction
    See expiration date on vial
  • Source
    Sheep were immunized with a recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.
  • Reactivity Supplier
    Human;Mouse;Rat;Dog
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CALR using anti-CALR antibody (A00894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALR antigen affinity purified polyclonal antibody (Catalog # A00894-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CALR at approximately 60KD. The expected band size for CALR is at 48KD.
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