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Western blot (WB) analysis of Smad1/5/9 antibody (Cat.-No.: AP20533PU-N) in extracts from HeLa cells.
Western blot (WB) analysis of Smad1/5/9 antibody (Cat.-No.: AP20533PU-N) in extracts from HeLa cells.
Western blot (WB) analysis of Smad1/5/9 antibody (Cat.-No.: AP20533PU-N) in extracts from HeLa cells.

SMAD1 (+SMAD5/9) Rabbit Polyclonal Antibody

AP20533PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMAD1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SMAD1 (+SMAD5/9) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4086
  • Target name
    SMAD1
  • Target description
    SMAD family member 1
  • Target synonyms
    BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1, mothers against decapentaplegic homolog 1, MAD, mothers against decapentaplegic homolog 1, Mad-related protein 1, SMAD, mothers against DPP homolog 1, TGF-beta signaling protein 1, mothers against DPP homolog 1, transforming growth factor-beta signaling protein 1
  • Host
    Rabbit
  • Protein IDQ15797
  • Protein Name
    Mothers against decapentaplegic homolog 1
  • Scientific Description
    SMAD1 (+SMAD5/9) rabbit polyclonal antibody, Aff - Purified
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMAD1 using anti-SMAD1 antibody (A00728-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse kidney tissue lysate, Lane 5: mouse testis tissue lysate, Lane 6: human Hela cell lysate, Lane 7: human A375 cell lysate, Lane 8: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (Catalog # A00728-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD1 at approximately 52,60KD. The expected band size for SMAD1 is at 48, 52, 60KD.
Product group Antibodies
Boster Bio
Anti-SMAD1/SMAD5 Antibody Picoband(r)A00728-1-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetSMAD1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Smad1 Antibody130-00004
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetSMAD1
  • SizePrice
Product group Antibodies
Biosynth
SMAD1 antibody10R-1435
  • SizePrice
Product group Antibodies
Biomatik
SMAD1 Polyclonal AntibodyCAC14179
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetSMAD1
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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad1 + Smad5 Antibody(bs-1619R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
Smad1 Polyclonal AntibodyBS-1619R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetSMAD1
  • SizePrice
Western Blot analysis of Jurkat cells using Smad1 Polyclonal Antibody
Product group Antibodies
Cusabio
SMAD1 AntibodyCSB-PA004104
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSMAD1
  • SizePrice
Anti-Smad1 Antibody
Product group Antibodies
Antibodies.com
Anti-Smad1 AntibodyA95781
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
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Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.
Product group Antibodies
RevMAb Biosciences
anti-Phospho-Smad1 (Ser463/465) / Smad5 (Ser463/465) / Smad9 (Ser465/467), Rabbit Monoclonal (RM487)REV-31-1379-00
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ReactivityHuman, Mouse, Rat
TargetSMAD1
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LifeSpan BioSciences
SMAD1 Antibody (phospho-Ser214, Preservative Free)LS-C150314
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetSMAD1
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AKT1 Polyclonal AntibodyCAB11016
ReactivityHuman
TargetSMAD1
  • SizePrice