More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SMAD7 Antibody (Preservative Free)

SMAD7 Antibody (Preservative Free)

LS-C815436
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSMAD7
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMAD7 Antibody (Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4092
  • Target name
    SMAD7
  • Target description
    SMAD family member 7
  • Target synonyms
    CRCS3, MADH7, MADH8, mothers against decapentaplegic homolog 7, MAD (mothers against decapentaplegic, Drosophila) homolog 7, MAD homolog 8, Mothers against decapentaplegic, drosophila, homolog of, 7, SMAD, mothers against DPP homolog 7, hSMAD7, mothers against DPP homolog 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

Go to our frequently asked questions

Related products

Western Blot Positive WB detected in: HepG2 whole cell lysate, LO2 whole cell lysate All lanes: SMAD7 antibody at 2.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 24 kDa Observed band size: 47 kDa
Product group Antibodies
Cusabio
SMAD7 AntibodyCSB-PA021793LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman
TargetSMAD7
  • SizePrice
Product group Antibodies
GeneTex
SMAD7 antibodyGTX134232
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetSMAD7
  • SizePrice
Anti-Smad7 Antibody
Product group Antibodies
Antibodies.com
Anti-Smad7 AntibodyA28553
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
Biosynth
SMAD7 antibody70R-50031
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Mouse/Rat SMAD7 Antibody144-12343
ApplicationsImmunoFluorescence, Western Blot
TargetSMAD7
  • SizePrice
Immunohistochemical staining of human lung shows strong nuclear and cytoplasmic positivity in pneumocytes and macrophages.
Product group Antibodies
Atlas Antibodies
Anti-SMAD7 AntibodyHPA028897
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSMAD7
  • SizePrice
Figure 1. Western blot analysis of MADH7/SMAD7 using anti-MADH7/SMAD7 antibody (A00784-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADH7/SMAD7 antigen affinity purified polyclonal antibody (Catalog # A00784-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MADH7/SMAD7 at approximately 50KD. The expected band size for MADH7/SMAD7 is at 50KD.
Product group Antibodies
Boster Bio
Anti-MADH7/SMAD7 Antibody Picoband(r)A00784-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetSMAD7
  • SizePrice
Product group Antibodies
Biomatik
SMAD7 Polyclonal AntibodyCAC15698
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetSMAD7
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Smad7 Polyclonal Antibody, Unconjugated (bs-0566R) at 1:600 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
References
Bioss
Smad7 Polyclonal AntibodyBS-0566R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetSMAD7
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SMAD7 AntibodyLS-C832606
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetSMAD7
  • SizePrice