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ProductsBack/SMC5 (B-11) Alexa Fluor(r) 546

SMC5 (B-11) Alexa Fluor(r) 546

SC-393282AF546
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMC5
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    SMC5 (B-11) Alexa Fluor(r) 546
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 546
  • Gene ID23137
  • Target name
    SMC5
  • Target description
    structural maintenance of chromosomes 5
  • Target synonyms
    ATELS2, SMC5L1, structural maintenance of chromosomes protein 5, SMC protein 5, SMC-5, SMC5 structural maintenance of chromosomes 5-like 1, hSMC5
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMC5 using anti-SMC5 antibody (A01285). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC5 antigen affinity purified polyclonal antibody (Catalog # A01285) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC5 at approximately 130 kDa. The expected band size for SMC5 is at 129 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SMC5 Polyclonal Antibody, Unconjugated (bs-7729R) at 1:200 overnight at 4°C, DAB staining.
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Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate All lanes: SMC5 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 129 kDa Observed band size: 145 kDa
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Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (GTX115669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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