IHC-P analysis of human cerebellum tissue using GTX31147 SOD2 antibody [1H6]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
![IHC-P analysis of human kidney tissue using GTX31147 SOD2 antibody [1H6]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_1347_IHC-P_w_23060722_462.webp "IHC-P analysis of human kidney tissue using GTX31147 SOD2 antibody [1H6]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100")
![IHC-P analysis of human cervical carcinoma tissue using GTX31147 SOD2 antibody [1H6]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_1348_IHC-P_w_23060722_511.webp "IHC-P analysis of human cervical carcinoma tissue using GTX31147 SOD2 antibody [1H6]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200")
![IHC-P analysis of human liver tissue using GTX31147 SOD2 antibody [1H6]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_1349_IHC-P_w_23060722_851.webp "IHC-P analysis of human liver tissue using GTX31147 SOD2 antibody [1H6]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200")
![IP analysis of HepG2 cell lysates using GTX31147 SOD2 antibody [1H6]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_1453_IP_w_23060722_447.webp "IP analysis of HepG2 cell lysates using GTX31147 SOD2 antibody [1H6]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:500")
![WB analysis of 50 ug of various samples using GTX31147 SOD2 antibody [1H6]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_1855_WB_w_23060722_338.webp "WB analysis of 50 ug of various samples using GTX31147 SOD2 antibody [1H6]. Dilution : 1:500")
![WB analysis of whole cell extracts (30 microg lysate) of HepG2 (Lane 1), Jurkat (Lane 2), K562 (Lane 3), HeLa (Lane 4), U-87 MG (Lane 5), COLO 205 (Lane 6), HEK293 (Lane 7) and HUVEC (Lane 8) using GTX31147 SOD2 antibody [1H6]. Dilution : 1-2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_1856_WB_w_23060722_667.webp "WB analysis of whole cell extracts (30 microg lysate) of HepG2 (Lane 1), Jurkat (Lane 2), K562 (Lane 3), HeLa (Lane 4), U-87 MG (Lane 5), COLO 205 (Lane 6), HEK293 (Lane 7) and HUVEC (Lane 8) using GTX31147 SOD2 antibody [1H6]. Dilution : 1-2 microg/ml")
![ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton? X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_763_ICC-IF_w_23060722_949.webp "ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton? X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA incubated for 3 hours at room temperature")
![ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_764_ICC-IF_w_23060722_228.webp "ICC/IF analysis of HeLa cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC in a humidified chamber")
![ICC/IF analysis of HUVEC cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX31147/GTX31147_765_ICC-IF_w_23060722_797.webp "ICC/IF analysis of HUVEC cells using GTX31147 SOD2 antibody [1H6]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC in a humidified chamber")
IHC-P analysis of human cerebellum tissue using GTX31147 SOD2 antibody [1H6]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
SOD2 antibody [1H6]
GTX31147
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSOD2
Overview
- SupplierGeneTex
- Product NameSOD2 antibody [1H6]
- Delivery Days Customer9
- Application Supplier NoteWB: 1-2 microg/mL. ICC/IF: 2 microg/ml. IHC-P: 1:100. IP: 2 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1H6
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6648
- Target nameSOD2
- Target descriptionsuperoxide dismutase 2
- Target synonymsGC1, GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD, lncRNA-GC1, superoxide dismutase [Mn], mitochondrial, Mn superoxide dismutase, epididymis secretory sperm binding protein, gastric cancer-associated lncRNA 1, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial
- HostMouse
- IsotypeIgG
- Protein IDP04179
- Protein NameSuperoxide dismutase [Mn], mitochondrial
- Scientific DescriptionThis gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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