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ProductsBack/SOS1 Mouse Monoclonal Antibody [Clone ID: SOS-1]
Western Blotting analysis (reducing conditions) of human Sos using anti-Sos (SOS-01). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line;Lane 2: RAJI human Burkitt lymphoma cell line
Western Blotting analysis (reducing conditions) of human Sos using anti-Sos (SOS-01). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line;Lane 2: RAJI human Burkitt lymphoma cell line
Western Blotting analysis (reducing conditions) of human Sos using anti-Sos (SOS-01). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line;Lane 2: RAJI human Burkitt lymphoma cell line

SOS1 Mouse Monoclonal Antibody [Clone ID: SOS-1]

AM03036PU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetSOS1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SOS1 Mouse Monoclonal Antibody [Clone ID: SOS-1]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SOS-1
  • Gene ID6654
  • Target name
    SOS1
  • Target description
    SOS Ras/Rac guanine nucleotide exchange factor 1
  • Target synonyms
    GF1, GGF1, GINGF, HGF, NS4, SOS-1, son of sevenless homolog 1, gingival fibromatosis, hereditary, 1, guanine nucleotide exchange factor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ07889
  • Protein Name
    Son of sevenless homolog 1
  • Scientific Description
    SOS1 mouse monoclonal antibody, clone SOS-1, Aff - Purified
  • Reactivity
    Human, Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOS1 using anti-SOS1 antibody (A00837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOS1 antigen affinity purified polyclonal antibody (Catalog # A00837-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOS1 at approximately 170 kDa. The expected band size for SOS1 is at 152 kDa.
Product group Antibodies
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ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSOS1
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Product group Antibodies
RayBiotech
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ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetSOS1
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SOS1 Recombinant Antibody, Biotin ConjugatedBSM-61476R-BIOTIN
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ReactivityHuman, Mouse, Rat
TargetSOS1
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Western Blot Positive WB detected in: Rat brain tissue All lanes: SOS1 antibody at 4.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 153, 37 kDa Observed band size: 153 kDa
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TargetSOS1
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TargetSOS1
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TargetSOS1
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SOS1 antibody detects SOS1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOS1 stained by SOS1 antibody (GTX133774) diluted at 1:500. Blue: Hoechst 33342 staining.
Product group Antibodies
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SOS1 antibodyGTX133774
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
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TargetSOS1
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Product group Antibodies
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TargetSOS1
  • SizePrice
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Anti-SOS1 AntibodyCAB3272
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TargetSOS1
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