ICC/IF analysis of C6 cells using GTX01528 SP1 antibody [GT1220]. Blue : DAPI
![ICC/IF analysis of NIH-3T3 cells using GTX01528 SP1 antibody [GT1220]. Blue : DAPI](https://www.genetex.com/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_ICCIF_w_23053121_871.webp "ICC/IF analysis of NIH-3T3 cells using GTX01528 SP1 antibody [GT1220]. Blue : DAPI")
![IHC-P analysis of rat spleen tissue section using GTX01528 SP1 antibody [GT1220]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_IHC-P_1_w_23053121_526.webp "IHC-P analysis of rat spleen tissue section using GTX01528 SP1 antibody [GT1220]. Dilution : 1:100")
![IHC-P analysis of mouse kidney tissue section using GTX01528 SP1 antibody [GT1220]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_IHC-P_w_23053121_268.webp "IHC-P analysis of mouse kidney tissue section using GTX01528 SP1 antibody [GT1220]. Dilution : 1:100")
![WB analysis of various samples using GTX01528 SP1 antibody [GT1220]. Dilution : 1:1000 Loading : 25 microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_WB_w_23053121_631.webp "WB analysis of various samples using GTX01528 SP1 antibody [GT1220]. Dilution : 1:1000 Loading : 25 microg")
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT1220] (GTX01528) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01528/GTX01528_4000000128_20200410_WB_w_23053121_101.webp "Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT1220] (GTX01528) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT1220] (GTX01528) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01528/GTX01528_4000000128_20200417_WB_shRNA_watermark_w_23053121_180.webp "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT1220] (GTX01528) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
ICC/IF analysis of C6 cells using GTX01528 SP1 antibody [GT1220]. Blue : DAPI
SP1 antibody [GT1220]
GTX01528
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSP1
Overview
- SupplierGeneTex
- Product NameSP1 antibody [GT1220] - KO/KD Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1220
- ConjugateUnconjugated
- Gene ID6667
- Target nameSP1
- Target descriptionSp1 transcription factor
- Target synonymsspecificity protein 1; transcription factor Sp1
- HostRabbit
- IsotypeIgG
- Protein IDP08047
- Protein NameTranscription factor Sp1
- Scientific DescriptionThe protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203