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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SP1 antibody

GTX110593
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SP1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.22 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6667
  • Target name
    SP1
  • Target description
    Sp1 transcription factor
  • Target synonyms
    transcription factor Sp1, specificity protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08047
  • Protein Name
    Transcription factor Sp1
  • Scientific Description
    Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu R, Lin H, Zhang W, et al. Cooperation of long noncoding RNA LOC100909675 and transcriptional regulator CTCF modulates Cdk1 transcript to control astrocyte proliferation. J Biol Chem. 2023,299(9):105153. doi: 10.1016/j.jbc.2023.105153
    Read this paper
  • Moon SJ, Choi HJ, Kye YH, et al. CTTN Overexpression Confers Cancer Stem Cell-like Properties and Trastuzumab Resistance via DKK-1/WNT Signaling in HER2 Positive Breast Cancer. Cancers (Basel). 2023,15(4). doi: 10.3390/cancers15041168
    Read this paper
  • Li Y, Wang Y, Gao L, et al. Betulinic acid self-assembled nanoparticles for effective treatment of glioblastoma. J Nanobiotechnology. 2022,20(1):39. doi: 10.1186/s12951-022-01238-7
    Read this paper
  • González-González L, Gallego-Gutiérrez H, Martin-Tapia D, et al. ZO-2 favors Hippo signaling, and its re-expression in the steatotic liver by AMPK restores junctional sealing. Tissue Barriers. 2022,10(2):1994351. doi: 10.1080/21688370.2021.1994351
    Read this paper
  • Liu L, Deng Y, Zheng Z, et al. Hsp90 Inhibitor STA9090 Sensitizes Hepatocellular Carcinoma to Hyperthermia-Induced DNA Damage by Suppressing DNA-PKcs Protein Stability and mRNA Transcription. Mol Cancer Ther. 2021,20(10):1880-1892. doi: 10.1158/1535-7163.MCT-21-0215
    Read this paper
  • Lee J, Jung E, Gestoso K, et al. ZEB1 Mediates Fibrosis in Corneal Endothelial Mesenchymal Transition Through SP1 and SP3. Invest Ophthalmol Vis Sci. 2020,61(8):41. doi: 10.1167/iovs.61.8.41
    Read this paper
  • Chien SY, Tsai CH, Liu SC, et al. Noggin Inhibits IL-1β and BMP-2 Expression, and Attenuates Cartilage Degeneration and Subchondral Bone Destruction in Experimental Osteoarthritis. Cells. 2020,9(4). doi: 10.3390/cells9040927
    Read this paper
  • Seira N, Yamagata K, Fukushima K, et al. Cellular density-dependent increases in HIF-1α compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region. Pharmacol Res Perspect. 2018,6(6):e00441. doi: 10.1002/prp2.441
    Read this paper
  • Carrasco-Torres G, Monroy-Ramírez HC, Martínez-Guerra AA, et al. Quercetin Reverses Rat Liver Preneoplastic Lesions Induced by Chemical Carcinogenesis. Oxid Med Cell Longev. 2017,2017:4674918. doi: 10.1155/2017/4674918
    Read this paper

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Figure 1. Western blot analysis of SP1 using anti-SP1 antibody (A00110-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP1 antigen affinity purified polyclonal antibody (Catalog # A00110-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP1 at approximately 90 kDa. The expected band size for SP1 is at 81 kDa.
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