ICC/IF analysis of PFA-fixed HeLa cells using GTX01012 SP1 antibody [JF0950]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS
![ICC/IF analysis of PFA-fixed MCF-7 cells using GTX01012 SP1 antibody [JF0950]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS](https://www.genetex.com/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_ICC-IF_171_w_23053121_394.webp "ICC/IF analysis of PFA-fixed MCF-7 cells using GTX01012 SP1 antibody [JF0950]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS")
![ICC/IF analysis of PFA-fixed SW480 cells using GTX01012 SP1 antibody [JF0950]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS](https://www.genetex.com/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_ICC-IF_172_w_23053121_883.webp "ICC/IF analysis of PFA-fixed SW480 cells using GTX01012 SP1 antibody [JF0950]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS")
![IHC-P analysis of human breast carcinoma tissue section using GTX01012 SP1 antibody [JF0950].](https://www.genetex.com/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_IHC-P_173_w_23053121_640.webp "IHC-P analysis of human breast carcinoma tissue section using GTX01012 SP1 antibody [JF0950].")
![IHC-P analysis of human colon cancer tissue section using GTX01012 SP1 antibody [JF0950].](https://www.genetex.com/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_IHC-P_174_w_23053121_883.webp "IHC-P analysis of human colon cancer tissue section using GTX01012 SP1 antibody [JF0950].")
![IHC-P analysis of human kidney tissue section using GTX01012 SP1 antibody [JF0950].](https://www.genetex.com/upload/website/prouct_img/normal/GTX01012/GTX01012_20200303_IHC-P_175_w_23053121_873.webp "IHC-P analysis of human kidney tissue section using GTX01012 SP1 antibody [JF0950].")
![Whole cell extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01012/GTX01012_HJ1121_20200214_WB_2_w_23053121_341.webp "Whole cell extract (30 microg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01012/GTX01012_HJ1121_20200214_WB_w_23053121_165.webp "Various whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01012/GTX01012_HJ1121_20200306_WB_shRNA_watermark_w_23053121_388.webp "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [JF0950] (GTX01012) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
ICC/IF analysis of PFA-fixed HeLa cells using GTX01012 SP1 antibody [JF0950]. Green : primary antibody Blue : DAPI Permeabilization : 0.25% Triton X-100 in PBS
SP1 antibody [JF0950]
GTX01012
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSP1
Overview
- SupplierGeneTex
- Product NameSP1 antibody [JF0950] - Recombinant antibody, KO/KD Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. WB: 1:1000-1:2000. ICC/IF: 1:100-1:500. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDJF0950
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6667
- Target nameSP1
- Target descriptionSp1 transcription factor
- Target synonymsspecificity protein 1; transcription factor Sp1
- HostRabbit
- IsotypeIgG
- Protein IDP08047
- Protein NameTranscription factor Sp1
- Scientific DescriptionThe protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203