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The data was published in the journal BMC Dev Biol in 2015. PMID: 25879198
The data was published in the journal BMC Dev Biol in 2015. PMID: 25879198
The data was published in the journal BMC Dev Biol in 2015. PMID: 25879198

Sp7 antibody, C-term

GTX50962
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSP7
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Overview

  • Supplier
    GeneTex
  • Product Name
    Sp7 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. IHC-P: 2-10 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID121340
  • Target name
    SP7
  • Target description
    Sp7 transcription factor
  • Target synonyms
    OI11; OI12; osterix; OSX; transcription factor Sp7; zinc finger protein osterix
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDD2
  • Protein Name
    Transcription factor Sp7
  • Scientific Description
    This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.[provided by RefSeq, Jul 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Titanium Surface Properties Influence the Biological Activity and FasL Expression of Craniofacial Stromal Cells. Conserva E et al., 2019, Stem Cells Int
    Read more
  • Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. Pisciotta A et al., 2015 Mar 14, BMC Dev Biol
    Read more
  • Ferutinin promotes proliferation and osteoblastic differentiation in human amniotic fluid and dental pulp stem cells. Zavatti M et al., 2013 May 30, Life Sci
    Read more
  • Ossification process involving the human thoracic ligamentum flavum: role of transcription factors. Uchida K et al., 2011, Arthritis Res Ther
    Read more
  • Human amniotic fluid stem cells seeded in fibroin scaffold produce in vivo mineralized matrix. Maraldi T et al., 2011 Nov, Tissue Eng Part A
    Read more
  • Consistent osteoblastic differentiation of human mesenchymal stem cells with bone morphogenetic protein 4 and low serum. Cordonnier T et al., 2011 Mar, Tissue Eng Part C Methods
    Read more

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Figure 1. Western blot analysis of Sp7/Osterix using anti-Sp7/Osterix antibody (A02077-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human MDA-MB453 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates. Lane 7: rat lung tissue lysates, Lane 8: rat thymus tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sp7/Osterix antigen affinity purified polyclonal antibody (Catalog # A02077-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sp7/Osterix at approximately 45KD. The expected band size for Sp7/Osterix is at 45KD.
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