Immunofluorescence analysis of paraformaldehyde-fixed A431, using SPHK1(GTX104724) antibody at 1:200 dilution.
![Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SPHK1 antibody [C3], C-term (GTX104724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104724/GTX104724_45049_20230609_WB_shRNA_watermark_23061400_585.webp "Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SPHK1 antibody [C3], C-term (GTX104724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SPHK1 antibody [C3], C-term (GTX104724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104724/GTX104724_45091_20230707_WB_TPM_watermark_25042920_317.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SPHK1 antibody [C3], C-term (GTX104724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Immunofluorescence analysis of paraformaldehyde-fixed A431, using SPHK1(GTX104724) antibody at 1:200 dilution.
SPHK1 antibody [C3], C-term
GTX104724
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSPHK1
Overview
- SupplierGeneTex
- Product NameSPHK1 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.17 mg/ml
- ConjugateUnconjugated
- Gene ID8877
- Target nameSPHK1
- Target descriptionsphingosine kinase 1
- Target synonymsSPHK, sphingosine kinase 1, SK 1, SPK 1, acetyltransferase SPHK1
- HostRabbit
- IsotypeIgG
- Protein IDQ9NYA1
- Protein NameSphingosine kinase 1
- Scientific DescriptionSphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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