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Goat anti-SPHK1

EB08409
Everest Biotech
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetSPHK1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SPHK1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID8877
  • Target name
    SPHK1
  • Target description
    sphingosine kinase 1
  • Target synonyms
    acetyltransferase SPHK1; SK 1; sphingosine kinase 1; SPHK; SPK 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_068807.2; NP_892010.2; NP_001136073.1. GeneIDs all Nonhuman: 20698 (mouse); 170897 (rat);. Purification: Antigen affinity purified. Names and symbols: SPHK1; sphingosine kinase 1; SPHK; SK1; SKase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (Catalog # A01390-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPHK1 at approximately 45-50 kDa. The expected band size for SPHK1 is at 45-50 kDa.
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