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SPHK1 antibody

20R-SR024
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    SPHK1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Mouse heart lysates probed with SPHK1 Polyclonal Antibody, unconjugated (bs-2652R) at 1:30 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n
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References
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Figure 1. Western blot analysis of SPHK1 using anti-SPHK1 antibody (A01390-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK1 antigen affinity purified polyclonal antibody (Catalog # A01390-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPHK1 at approximately 45-50 kDa. The expected band size for SPHK1 is at 45-50 kDa.
Product group Antibodies
Boster Bio
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