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ProductsBack/SPI1 / PU.1 Antibody (clone 5C7)

SPI1 / PU.1 Antibody (clone 5C7)

LS-C169103
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetSPI1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SPI1 / PU.1 Antibody (clone 5C7)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6688
  • Target name
    SPI1
  • Target description
    Spi-1 proto-oncogene
  • Target synonyms
    31 kDa transforming protein; hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SPI-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1; transcription factor PU.1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PU.1/SPI1 using anti-PU.1/SPI1 antibody (A01116-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PU.1/SPI1 antigen affinity purified polyclonal antibody (Catalog # A01116-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PU.1/SPI1 at approximately 40 kDa. The expected band size for PU.1/SPI1 is at 40 kDa.
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