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FACS analysis of HeLa cells using GTX35091 SPTBN2 antibody [SPTBN2/1584]. Blue : Primary antibody Red : Isotype control
FACS analysis of HeLa cells using GTX35091 SPTBN2 antibody [SPTBN2/1584]. Blue : Primary antibody Red : Isotype control
FACS analysis of HeLa cells using GTX35091 SPTBN2 antibody [SPTBN2/1584]. Blue : Primary antibody Red : Isotype control

SPTBN2 antibody [SPTBN2/1584]

GTX35091
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
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Product group Antibodies
TargetSPTBN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SPTBN2 antibody [SPTBN2/1584]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-2microg/ml. ICC/IF: 1-2microg/ml. IHC-P: 1-2microg/ml for 30 minutes at RT. FACS: 1-2microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SPTBN2/1584
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6712
  • Target name
    SPTBN2
  • Target description
    spectrin beta, non-erythrocytic 2
  • Target synonyms
    beta-III spectrin; glutamate transporter EAAT4-associated protein 41; GTRAP41; SCA5; SCAR14; spectrin beta chain, brain 2; spectrin beta chain, non-erythrocytic 2; spectrin beta III sigma 2; spectrin, non-erythroid beta chain 2; spinocerebellar ataxia 5 protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO15020
  • Protein Name
    Spectrin beta chain, non-erythrocytic 2
  • Scientific Description
    Spectrins are principle components of a cells membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPTBN2 using anti-SPTBN2 antibody (A05492). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPTBN2 antigen affinity purified polyclonal antibody (Catalog # A05492) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPTBN2 at approximately 271 kDa. The expected band size for SPTBN2 is at 271 kDa.
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