WB (peptide competition) analysis of 3T3L1 cells stimulated with 20 ng/mL LIF for 10 minutes (Lane 2-5) using GTX24740 STAT3 (phospho Tyr705) antibody prior incubated with the phosphopeptide immunogen (Lane 2), a generic phosphotyrosine-containing peptide (Lane 3), or the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of 3T3L1 cells stimulated with 20 ng/mL LIF for 10 minutes (Lane 2-5) using GTX24740 STAT3 (phospho Tyr705) antibody prior incubated with the phosphopeptide immunogen (Lane 2), a generic phosphotyrosine-containing peptide (Lane 3), or the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
STAT3 (phospho Tyr705) antibody - Orthogonal Validated
GTX24740
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAT3
Overview
- SupplierGeneTex
- Product NameSTAT3 (phospho Tyr705) antibody - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID6774
- Target nameSTAT3
- Target descriptionsignal transducer and activator of transcription 3
- Target synonymsacute-phase response factor; ADMIO; ADMIO1; APRF; DNA-binding protein APRF; HIES; signal transducer and activator of transcription 3
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203