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ICC/IF analysis of PFA-fixed RBL cells using GTX54768 STIM2 antibody. Panel A,D : Primary antibody Panel B,E : DNA dye Hoechst 33342 Panel C,F : Merged images of panels A and B Dilution : 1:100
ICC/IF analysis of PFA-fixed RBL cells using GTX54768 STIM2 antibody. Panel A,D : Primary antibody Panel B,E : DNA dye Hoechst 33342 Panel C,F : Merged images of panels A and B Dilution : 1:100
ICC/IF analysis of PFA-fixed RBL cells using GTX54768 STIM2 antibody. Panel A,D : Primary antibody Panel B,E : DNA dye Hoechst 33342 Panel C,F : Merged images of panels A and B Dilution : 1:100

STIM2 antibody

GTX54768
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSTIM2
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Overview

  • Supplier
    GeneTex
  • Product Name
    STIM2 antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57620
  • Target name
    STIM2
  • Target description
    stromal interaction molecule 2
  • Target synonyms
    stromal interaction molecule 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P246
  • Protein Name
    Stromal interaction molecule 2
  • Scientific Description
    This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STIM2 using anti-STIM2 antibody (A02345-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM2 antigen affinity purified polyclonal antibody (Catalog # A02345-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIM2 at approximately 98,100 kDa. The expected band size for STIM2 is at 84 kDa.
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