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ProductsBack/SUCLA2 Antibody - N-terminal region (ARP64676_P050)

SUCLA2 Antibody - N-terminal region (ARP64676_P050)

ORB331292
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Yeast, Zebra Fish
TargetSUCLA2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    SUCLA2 Antibody - N-terminal region (ARP64676_P050)
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8803
  • Target name
    SUCLA2
  • Target description
    succinate-CoA ligase ADP-forming subunit beta
  • Target synonyms
    A-BETA, A-SCS, LINC00444, MTDPS5, SCS-betaA, succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, ATP-specific succinyl-CoA synthetase subunit beta, ATP-specific succinyl-CoA synthetase, beta subunit, long intergenic non-protein coding RNA 444, mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta, renal carcinoma antigen NY-REN-39, succinate-CoA ligase ADP-forming beta subunit, succinate-CoA ligase beta subunit, succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial, succinyl-CoA synthetase beta-A chain
  • Host
    Rabbit
  • Protein IDQ9P2R7
  • Protein Name
    Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
  • Scientific Description
    Rabbit polyclonal antibody to SUCLA2
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Yeast, Zebra Fish
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SUCLA2 using anti-SUCLA2 antibody (A04807-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLA2 antigen affinity purified polyclonal antibody (Catalog # A04807-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUCLA2 at approximately 48 kDa. The expected band size for SUCLA2 is at 48 kDa.
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