Suppressor of Fused antibody [HL3375] detects Suppressor of Fused protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Suppressor of Fused stained by Suppressor of Fused antibody [HL3375] (GTX641195) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3375] (GTX641195) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641195/GTX641195_T-45572_20241220_WB_24122400_797.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3375] (GTX641195) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3375] (GTX641195) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641195/GTX641195_T-45572_20241220_WB_R_24122400_320.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3375] (GTX641195) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Suppressor of Fused antibody [HL3375] detects Suppressor of Fused protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Suppressor of Fused stained by Suppressor of Fused antibody [HL3375] (GTX641195) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Suppressor of Fused antibody [HL3375]
GTX641195
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetSUFU
Overview
- SupplierGeneTex
- Product NameSuppressor of Fused antibody [HL3375]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3375
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID51684
- Target nameSUFU
- Target descriptionSUFU negative regulator of hedgehog signaling
- Target synonymsBCNS2, JBTS32, PRO1280, SUFUH, SUFUXL, suppressor of fused homolog, negative regulator of hedgehog signaling
- HostRabbit
- IsotypeIgG
- Protein IDQ9UMX1
- Protein NameSuppressor of fused homolog
- Scientific DescriptionThe Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
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![Rat whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3374] (GTX641194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641194/GTX641194_T-45572_20241025_WB_R_24103022_629.webp)
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