
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using SYK(GTX107459) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Syk antibody [N1C1]
GTX107459
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSYK
Overview
- SupplierGeneTex
- Product NameSyk antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.02 mg/ml
- ConjugateUnconjugated
- Gene ID6850
- Target nameSYK
- Target descriptionspleen associated tyrosine kinase
- Target synonymsIMD82, p72-Syk, tyrosine-protein kinase SYK, spleen tyrosine kinase
- HostRabbit
- IsotypeIgG
- Protein IDP43405
- Protein NameTyrosine-protein kinase SYK
- Scientific DescriptionThis gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N1C1] (GTX107459) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N1C1] (GTX107459) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107459/GTX107459_39939_20190823_WB_w_23060120_817.webp)
![SYK antibody [N1C1] detects SYK protein on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. SYK antibody [N1C1] (GTX107459) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min SYK antibody [N1C1] detects SYK protein on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. SYK antibody [N1C1] (GTX107459) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX107459/GTX107459_39939_IHC_M_w_23060120_151.webp)



![SYK antibody [N2C2], Internal detects SYK protein by western blot analysis. A. 30 μg mouse BMDM (bone marrow-derived macrophage) cells B. 30 μg mouse Syk null cells 10% SDS-PAGE SYK antibody [N2C2], Internal (GTX100748) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100748/GTX100748_40051_WB_M_w_23060100_280.webp)
![Syk antibody [GT351] detects Syk protein at cytoplasm and cell membrane by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syk stained by Syk antibody [GT351] (GTX633910) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633910/GTX633910_43314_20190109_ICC_IF_w_23061202_109.webp)

