WB (peptide competition) analysis of Jurkat cells treated with 10 mM H2O2 for 3 minutes (Lane 2-6) using GTX12862 Syk (phospho Tyr323) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with lambda phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of Jurkat cells treated with 10 mM H2O2 for 3 minutes (Lane 2-6) using GTX12862 Syk (phospho Tyr323) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with lambda phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.
Syk (phospho Tyr323) antibody
GTX12862
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSYK
Overview
- SupplierGeneTex
- Product NameSyk (phospho Tyr323) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID6850
- Target nameSYK
- Target descriptionspleen associated tyrosine kinase
- Target synonymsIMD82; p72-Syk; spleen tyrosine kinase; tyrosine-protein kinase SYK
- HostRabbit
- IsotypeIgG
- Protein IDP43405
- Protein NameTyrosine-protein kinase SYK
- Scientific DescriptionThis gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203