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Synaptophysin Antibody (YA043, Rabbit)

HY-P80354
MedChem Express
TargetSYP
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Synaptophysin Antibody (YA043, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6855
  • Target name
    SYP
  • Target description
    synaptophysin
  • Target synonyms
    major synaptic vesicle protein P38; MRX96; MRXSYP; synaptophysin; XLID96
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Synaptophysin Antibody (YA043) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Synaptophysin.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Lane 1:Human Cerebrum lysates; Lane 2: PC-12 cell lysates probed with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
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Figure 1. Western blot analysis of SYP using anti-SYP antibody (A05049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYP antigen affinity purified polyclonal antibody (Catalog # A05049) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
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GeneTex
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