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ProductsBack/Syntaxin antibody [4H256]
The data was published in the journal PLoS One in 2015.PMID: 26431040
The data was published in the journal PLoS One in 2015.PMID: 26431040
The data was published in the journal PLoS One in 2015.PMID: 26431040

Syntaxin antibody [4H256]

GTX18010
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSTX1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    Syntaxin antibody [4H256]
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4H256
  • Conjugate
    Unconjugated
  • Gene ID6804
  • Target name
    STX1A
  • Target description
    syntaxin 1A
  • Target synonyms
    HPC-1; neuron-specific antigen HPC-1; P35-1; STX1; SYN1A; syntaxin 1A (brain); syntaxin-1A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16623
  • Protein Name
    Syntaxin-1A
  • Scientific Description
    This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The Depolarization-Evoked, Ca(2+)-Dependent Release of Exosomes From Mouse Cortical Nerve Endings: New Insights Into Synaptic Transmission. Olivero G et al., 2021, Front Pharmacol
    Read more
  • Physiological Roles of Calpain 1 Associated to Multiprotein NMDA Receptor Complex. Averna M et al., 2015, PLoS One
    Read more

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Figure 1. Western blot analysis of Syntaxin 1a using anti-Syntaxin 1a antibody (PB9408). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 1a antigen affinity purified polyclonal antibody (Catalog # PB9408) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 1a at approximately 37 kDa. The expected band size for Syntaxin 1a is at 33 kDa.
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