Paraformaldehyde-fixed, paraffin embedded mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Syntaxin 1a(Ser188) Polyclonal Antibody, Unconjugated (bs-10315R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Syntaxin 1a(Ser188) Polyclonal Antibody, Unconjugated (bs-10315R) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining.
phospho-Syntaxin 1a (Ser188) Polyclonal Antibody
BS-10315R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
TargetSTX1A
Overview
- SupplierBioss
- Product Namephospho-Syntaxin 1a (Ser188) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID6804
- Target nameSTX1A
- Target descriptionsyntaxin 1A
- Target synonymsHPC-1, P35-1, STX1, SYN1A, syntaxin-1A, neuron-specific antigen HPC-1, syntaxin 1A (brain)
- HostRabbit
- IsotypeIgG
- Protein IDQ16623
- Protein NameSyntaxin-1A
- ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC41116161
Datasheet
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![Syntaxin 1a antibody [N1], N-term detects Syntaxin 1a protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Syntaxin 1a protein stained by Syntaxin 1a antibody [N1], N-term (GTX106365) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX106365/GTX106365_39715_20170727_IFA_R_w_23060120_748.webp)
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