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TAGLN Antibody Pair

CSB-EAP00804
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityAvian, Bovine, Canine, Equine, Guinea Pig, Goat, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetTAGLN
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Overview

  • Supplier
    Cusabio
  • Product Name
    TAGLN Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.8ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID6876
  • Target name
    TAGLN
  • Target description
    transgelin
  • Target synonyms
    22 kDa actin-binding protein; epididymis secretory sperm binding protein; SM22; SM22-alpha; SMCC; smooth muscle protein 22-alpha; TAGLN1; transgelin; transgelin variant 2; WS3-10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01995
  • Protein Name
    Transgelin
  • Reactivity
    Avian, Bovine, Canine, Equine, Guinea Pig, Goat, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of TAGLN/Transgelin using anti-TAGLN/Transgelin antibody (A03962-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAGLN/Transgelin antigen affinity purified polyclonal antibody (Catalog # A03962-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAGLN/Transgelin at approximately 22KD. The expected band size for TAGLN/Transgelin is at 22KD.
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