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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody [HL1703] (GTX637302) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody [HL1703] (GTX637302) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody [HL1703] (GTX637302) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

TAL1 antibody [HL1703]

GTX637302
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetTAL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TAL1 antibody [HL1703]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1703
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6886
  • Target name
    TAL1
  • Target description
    TAL bHLH transcription factor 1, erythroid differentiation factor
  • Target synonyms
    SCL, TCL5, bHLHa17, tal-1, T-cell acute lymphocytic leukemia protein 1, T-cell acute lymphocytic leukemia 1, T-cell leukemia/lymphoma protein 5, class A basic helix-loop-helix protein 17, stem cell protein, tal-1 product
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17542
  • Protein Name
    T-cell acute lymphocytic leukemia protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Tal1 using anti-Tal1 antibody (A00944-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tal1 antigen affinity purified polyclonal antibody (Catalog # A00944-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tal1 at approximately 39KD. The expected band size for Tal1 is at 34KD.
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