![Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Aμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Data provided byA?YCharOSA?Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology. Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Aμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Data provided byA?YCharOSA?Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630196/GTX630196_41505_20220805_WB_KO_22080923_561.webp)
Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Aμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Data provided byA?YCharOSA?Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
TDP43 antibody [GT225]
GTX630196
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTARDBP
Overview
- SupplierGeneTex
- Product NameTDP43 antibody [GT225]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT225
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID23435
- Target nameTARDBP
- Target descriptionTAR DNA binding protein
- Target synonymsALS10, TDP-43, TAR DNA-binding protein 43, TAR DNA-binding protein-43
- HostMouse
- IsotypeIgG2b
- Protein IDQ13148
- Protein NameTAR DNA-binding protein 43
- Scientific DescriptionHIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Worrall D, Ayoubi R, Fotouhi M, et al. The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence. F1000Res. 2023,12:277. doi: 10.12688/f1000research.131852.2Read this paper
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630196/GTX630196_41505_20160505_WB_w_23061202_169.webp)
![TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TDP43 antibody [GT225] (GTX630196) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TDP43 antibody [GT225] (GTX630196) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX630196/GTX630196_41505_20171020_IHC-P_R_w_23061202_187.webp)
![TARDBP antibody [GT225] detects TARDBP protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:1000 TARDBP antibody [GT225] detects TARDBP protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630196/GTX630196_41505_WB_R_w_23061202_706.webp)
![TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TDP43 antibody [GT225] (GTX630196) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TDP43 antibody [GT225] (GTX630196) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX630196/GTX630196_41505_20171020_IHC-P_M_w_23061202_628.webp)
![TARDBP antibody [GT225] detects TARDBP protein by western blot analysis. A. 30 μg BCL-1 whole cell lysate/extract B. 30 μg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:1000 TARDBP antibody [GT225] detects TARDBP protein by western blot analysis. A. 30 μg BCL-1 whole cell lysate/extract B. 30 μg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630196/GTX630196_41505_WB_M_w_23061202_373.webp)
![TARDBP antibody [GT225] validation by siRNA knock-down. Upperpanel: TARDBP antibody [GT225] GTX630196 Lower panel: GAPDH antibody (GTX100118) A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:3000 GAPDH antibody (GTX100118) dilution: 1:10000 TARDBP antibody [GT225] validation by siRNA knock-down. Upperpanel: TARDBP antibody [GT225] GTX630196 Lower panel: GAPDH antibody (GTX100118) A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells 10 % SDS-PAGE TARDBP antibody [GT225] (GTX630196) dilution: 1:3000 GAPDH antibody (GTX100118) dilution: 1:10000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630196/GTX630196_41505_WB_shRNA_watermark_w_23061202_694.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT225] (GTX630196) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630196/GTX630196_45252_20231201_WB_23120519_257.webp)



![Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Aμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT733] (GTX630197) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Data provided byA?YCharOSA?Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630197/GTX630197_41505_20220805_WB_KO_22080923_274.webp)
![TDP43 antibody [GT6310] detects TDP43 Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: TDP43 stained by TDP43 antibody [GT6310] (GTX633973) diluted at 1:250. Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 5 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633973/GTX633973_42730_20170829_IHC-Fr_M_w_23061202_828.webp)

