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ProductsBack/Thy1 Rat Monoclonal Antibody [Clone ID: 30-H12]
Immunofluorescent Staining: BALB/c splenocytes were double-stained with Rat anti-Mouse CD90.2-FITC and Rat anti-Mouse CD19-PE. Small lymphocytes were then gated and analyzed on a FACScan (TM) flowcytometer (BDIS, San Jose, CA). Amount Used: 0.3 ug/10e6 cells.
Immunofluorescent Staining: BALB/c splenocytes were double-stained with Rat anti-Mouse CD90.2-FITC and Rat anti-Mouse CD19-PE. Small lymphocytes were then gated and analyzed on a FACScan (TM) flowcytometer (BDIS, San Jose, CA). Amount Used: 0.3 ug/10e6 cells.
Immunofluorescent Staining: BALB/c splenocytes were double-stained with Rat anti-Mouse CD90.2-FITC and Rat anti-Mouse CD19-PE. Small lymphocytes were then gated and analyzed on a FACScan (TM) flowcytometer (BDIS, San Jose, CA). Amount Used: 0.3 ug/10e6 cells.

Thy1 Rat Monoclonal Antibody [Clone ID: 30-H12]

AM08055FC-N
OriGene
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetThy1
Price on request
500 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Thy1 Rat Monoclonal Antibody [Clone ID: 30-H12]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    30-H12
  • Gene ID21838
  • Target name
    Thy1
  • Target description
    thymus cell antigen 1, theta
  • Target synonyms
    CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2, thy-1 membrane glycoprotein, thy-1 antigen
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDP01831
  • Protein Name
    Thy-1 membrane glycoprotein
  • Scientific Description
    Thy1 rat monoclonal antibody, clone 30-H12, FITC
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rai thymus tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 22 kDa. The expected band size for CD90/Thy1 is at 18 kDa.
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